长爪沙鼠胃肠道Cajal间质细胞的形态学

doi:10.3969/j.issn.0529-1356.2013.01.018

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 93-96.doi: 10.3969/j.issn.0529-1356.2013.01.018

• 组织学胚胎学发育生物学 • 上一篇下一篇

长爪沙鼠胃肠道Cajal间质细胞的形态学

刘丽,孙海梅,吴波,周德山*

首都医科大学基础医学院组织学胚胎学教研室，北京 100069

收稿日期:2012-09-10 修回日期:2012-10-29 出版日期:2013-02-06 发布日期:2013-02-06
通讯作者: 周德山 E-mail:zhouds08@ccmu.edu.cn
基金资助:
国家自然科学基金资助项目;自然科学基金资助项目

Morphology of interstitial cells of Cajal in the gastrointestinal tract of Mongolian gerbil

LIU Li,SUN Hai-mei,WU Bo,ZHOU De-shan*

Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2012-09-10 Revised:2012-10-29 Online:2013-02-06 Published:2013-02-06

摘要/Abstract

摘要：

目的探讨长爪沙鼠胃肠道Cajal间质细胞（ICCs）的形态和分布规律。方法采用10只成年长爪沙鼠，体重50~70g，取胃、小肠、结肠制作冷冻切片，结合全层铺片的c-Kit免疫荧光染色。结果 ICCs呈网络状分布于整个胃肠道，不同部位ICCs的分布及形态有所不同。在胃底部，仅见肌内ICCs(ICC-IM），而在胃体和胃窦部除ICC-IM外，可见肌间ICCs(ICC-MY)分布在肌间神经丛周围；其细胞密度胃底ICC-IM最多，由胃底至胃窦逐渐减少，而ICC-MY由胃体至胃窦逐渐增多。在小肠可见ICC-IM, ICC-MY和深肌层ICCs(ICC-DMP)3个亚群，结肠管壁内也分布有ICC-IM、ICC-MY和黏膜下ICCs(ICC-SM)3个亚群。结论沙鼠可用于有关ICCs正常形态、结构及功能的研究。

关键词: Cajal间质细胞, Kit, 胃肠道, 免疫荧光, 长爪沙鼠

Abstract:

Objective To observe the distribution and morphological features of interstitial cells of Cajal (ICCs) in the gastrointestinal (GI) tract of Mongolian gerbil. Methods Whole GI tracts, including stomach, small and large intestines of ten adult Mongolian gerbils were removed and performed for C-kit immunocytochemical staining with whole-mount preparations and cryo-sections. Results c-Kit positive immunoreactivity cells distributed along the entire GI tract. Intramuscular ICC(ICC-IM) cells were only in the fundus of the stomach.Myenteric ICC(ICC-MY) profiels were encountered between the longitudinal and circular muscle layers of the corpus and antrum of the stomach. The density of ICC-IM profiels gradually decreased from the fundus to the antrum. Whereas, ICC-MY profiels gradually increased from the corpus to the antrum. In the small intestine, ICCs were often observed in three regions, i.e. deep muscular plexus ICC(ICC-DMP), ICC-IM and ICC-MY. While in the colon, ICCs were seen as ICC-IM, ICC-MY and Swbmucosal ICC(ICC-SM). Conclusion Mongolian gerbil may be used to research the morphology and function of ICCs.

Key words: Interstitial cells of Cajal, Kit, Gastrointestinal tract , Immunofluorescence, Mongolian gerbil

中图分类号:

R329

刘丽孙海梅吴波周德山*.

长爪沙鼠胃肠道Cajal间质细胞的形态学

[J]. 解剖学报, 2013, 44(1 ): 93-96.

LIU Li,SUN Hai-mei,WU Bo,ZHOU De-shan*. Morphology of interstitial cells of Cajal in the gastrointestinal tract of Mongolian gerbil[J]. AAS, 2013, 44(1 ): 93-96.

参考文献

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长爪沙鼠胃肠道Cajal间质细胞的形态学

Morphology of interstitial cells of Cajal in the gastrointestinal tract of Mongolian gerbil

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